Shared Lab Equipment

Upon entering, always become familar with the posted EHS Procedures & Response Guide which has specific information to respond to emergencies and make oneself aware of safety regulations.

Rules for booking equipment:
If you booked shared equipment without indicating your email address and lab affiliation your booking is invalid and will be removed.
People from outside of BCMP, non-quad laboratories can only book equipment 7 days in advance.

Liquid nitrogen is available at two locations, they are in the C Bldg, Rm. 310 and C Bldg, across from Rm. 207. Please follow all safety regulations, use the face shields that are available and bring your own cryo gloves.

Dark Room, located in C-211A.  Please check that no other person is using the dark room before entering.  In addition to the online sign-up, there is a flip sign outside the darkroom for you to indicate that it is occupied. Sign up using this calendar link: Sign up for Dark Room 211A

Typhoon 5 biomolecular imager (capable of both fluorescence imaging and phosphorimaging) – (BCMP and Cell Bio shared instrument)  Users must be trained.  Please contact Patrick Dennett for information and new instructions related to current work environment with COVID-19 safety considerations.

BD Accuri flow cytometer, located in LHRRB 209. All users must be trained by Emily Egan prior to use. Access to the sign-up calendar will be granted after training. Details about the machine can be found here.

SONY SH800 cell sorters (BCMP and Cell Bio shared instrument and Kruse Lab/BCMP shared instrument). These FACS machines can sort bacteria, yeast, and mammalian cells, All BCMP users must contact Anthony Banks for training and access to the calendar. Details about the machine can be found here.

The Center for Macromolecular Interactions (CMI) is a shared instrument facility in the BCMP department for biophysical characterization of macromolecules and their interactions.  Technologies at the CMI include:

GE Biacore T200 for surface plasmon resonance (SPR)
ForteBio Octet RED384 and BLItz for Biolayer Interferometry (BLI)
Microcal ITC200 for Isothermal Titration Calorimetry (ITC)
NanoTemper Monolith NT.115pico for Microscale Thermophoresis (MST)
Jasco J815 for Circular Dichroism (CD)
Analytical Size Exclusion Chromatography System with Wyatt Dawn Heleos II for multi-angle static light scattering (SEC-MALS)
Life Technologies Quant Studio 6 for Differential Scanning Fluorimetry (DSF)/Protein Thermal Shift Analysis and quantitative Real-time PCR

For more information visit cmi.hms.harvard.edu.

Optima LE-80 Ultra Centrifuge, located in C-310.  Rotors are in the -20 near the fume hood. The manual can be found here.
If you have questions about the instrument, please contact Steve Buratowski.

Optima XE-90 Ultracentrifuge, located in C-206.  The manual can be found here.

Optima LE-80K Ultra Centrifuge, located in C-302B.  The manual can be found here.

Optima L-90K Ultra Centrifuge, located in Mudd Corridor 216. Please remember to use the sign-in book.
The Optima L-90 Ultra Centrifuge user guide can be found by clicking here
 

RC-6C PLUS Centrifuge, located in C-368. There are two new rotors, a Fixed Angle; 4 x 1000ml, 9,500 rpm, SN 6111173 and a Carbon Fiber FS-18S-12 x 50, 18,000 rpm, SN 6112143. Please remember to use the sign-in book.
The user guide can be found by clicking here. If this centrifuge needs service please call 800-438-4851, and let Rob Loughran know.

Sorvall LYNX 6000 Centrifuge, located in LHRRB 216.  The manuals can be found here.

Sorvall LYNX 6000 Centrifuge, located in SGM 322.  The manuals can be found here.

If any of these centrifuges need service, please let Rob Loughran know.

Applied Biosystems 7500 FAST Real Time PCR System, located in C2-355 (Struhl lab). To reserve time on the machine, sign up using the Google calendar on the computer located in 325 (ask anyone in the lab for help).  Please limit your time to no more than 3 hours during weekday working hours (defined as 9:00 AM to 7:00 PM).  You may sign up for additional slots with the understanding that you may have to give them up upon request to anyone who hasn't used their PCR time allotment for the day.  In order to claim such slots, the person needing the slots must contact the person holding the extra slot(s) no later than 30 minutes prior to the start time of the slot.  On weekends, please be considerate and don't reserve an unreasonable amount of time.
If you must cancel a reserved time slot, please do it well IN ADVANCE.
IF YOU DON'T KNOW HOW TO USE THE MACHINE, please talk to Joe Geisberg, (C2-355).  DON'T attempt to use it if you haven't been trained.  BE VERY CAREFUL NOT TO CONTAMINATE THE INSTRUMENT.  Report any problems to Joe Geisberg, 
The Applied Biosystems 7500 FAST Real Time PCR System Guide can be found here.

NanoDrop, located in C Bldg, Rm 307, on the left of the north door.  Sign up for the instrument here. Instructions are displayed at the machine,  After hours, you need card access to get into C307. Please write to Rob Loughran and provide your ID# to get access.

Agilent 2100 Bioanalyzer located in C-310A. To sign up on the calendar follow the link below.
Sign-up for the Agilent 2100 Bioanalyzer
The Agilent 2100 Bioanalyzer user guide can be found on the bench.
If you have questions about the instrument, please contact Zarmik Moqtaderi, 

Contact Rob Loughran, if any of theshared equipment listed above requires service.

AUTOCLAVES & GLASSWASH

Please contact Rob Loughran if you need training on these machines.