Picture of Haribabu Arthanari

Haribabu Arthanari, Ph.D.

Associate Professor of Biological Chemistry and Molecular Pharmacology

We utilize a combination of techniques including NMR spectroscopy, NMR-based fragment and high throughput screening, and biophysical and cell-based assays to map hotspots in the interaction interface, to further understand the molecular mechanisms orchestrated by these interactions, and to identify disruptive inhibitors that may be developed into treatments for the related pathologies.

Haribabu Arthanari received his Bachelors in Chemistry from the Madras Christian College and his Masters in Chemistry from the Indian Institute of Technology (IIT)-Madras.  He did his graduate studies at Wesleyan University with Philip  Bolton and earned his PhD in 2004. He then joined the laboratory of Gerhard Wagner for his post-doctoral training. He was promoted to a lecturer in 2010 and moved to his independent position in 2016.

Research:

Protein-Protein Interactions (PPIs) is the Holy Grail of therapeutic intervention, offering a plethora of unique structural landscapes as potential targets. I use structure-guided approaches to characterize and validate these interactions in the context of disease models.  We utilize a combination of techniques including NMR spectroscopy, NMR-based fragment and high throughput screening, and biophysical and cell-based assays to map hotspots in the interaction interface, to further understand the molecular mechanisms orchestrated by these interactions, and to identify disruptive inhibitors that may be developed into treatments for the related pathologies.  Our current areas of focus are 1) the critical interactions between transcription factors and the general transcriptional machinery, including the Mediator complex, co-activators, and remodeling factors, and 2) translation initiation machinery demonstrated to be dysregulated in cancer disease states.  We are working on making use of NMR-derived metabolomics data in the identification of novel metabolite disease markers that in combination with cellular pathway analysis can be used to identify new potential therapeutic targets.  In order to facilitate our research goals, we also work on the development of new NMR methods for fragment screening, metabolite fingerprinting and protein-ligand interaction identification.  Our work on novel pulse sequences, pulse designs, labeling strategies and sampling schemes let us push the boundaries of NMR as a technique, allowing us to tackle larger systems by NMR.

Address: 

Longwood Center

LC-3311

360 Longwood Ave.

Boston, MA 02115

Publications View
Constitutively oxidized CXXC motifs within the CD3 heterodimeric ectodomains of the T cell receptor complex enforce the conformation of juxtaposed segments.
Authors: Authors: Brazin KN, Mallis RJ, Li C, Keskin DB, Arthanari H, Gao Y, Wu SL, Karger BL, Wagner G, Reinherz EL.
J Biol Chem
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Resonance assignments of the microtubule-binding domain of the C. elegans spindle and kinetochore-associated protein 1.
Authors: Authors: Boeszoermenyi A, Schmidt JC, Cheeseman IM, Oberer M, Wagner G, Arthanari H.
Biomol NMR Assign
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Selective methyl labeling of eukaryotic membrane proteins using cell-free expression.
Authors: Authors: Linser R, Gelev V, Hagn F, Arthanari H, Hyberts SG, Wagner G.
J Am Chem Soc
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Structure of the eukaryotic translation initiation factor eIF4E in complex with 4EGI-1 reveals an allosteric mechanism for dissociating eIF4G.
Authors: Authors: Papadopoulos E, Jenni S, Kabha E, Takrouri KJ, Yi T, Salvi N, Luna RE, Gavathiotis E, Mahalingam P, Arthanari H, Rodriguez-Mias R, Yefidoff-Freedman R, Aktas BH, Chorev M, Halperin JA, Wagner G.
Proc Natl Acad Sci U S A
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A new broadband homonuclear mixing pulse for NMR with low applied power.
Authors: Authors: Coote P, Leigh KE, Yu TY, Khaneja N, Wagner G, Arthanari H.
J Chem Phys
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Constitutively oxidized CXXC motifs within the CD3 heterodimeric ectodomains of the T cell receptor complex enforce the conformation of juxtaposed segments.
Authors: Authors: Brazin KN, Mallis RJ, Li C, Keskin DB, Arthanari H, Gao Y, Wu SL, Karger BL, Wagner G, Reinherz EL.
J Biol Chem
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Perspectives in magnetic resonance: NMR in the post-FFT era.
Authors: Authors: Hyberts SG, Arthanari H, Robson SA, Wagner G.
J Magn Reson
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The interaction between eukaryotic initiation factor 1A and eIF5 retains eIF1 within scanning preinitiation complexes.
Authors: Authors: Luna RE, Arthanari H, Hiraishi H, Akabayov B, Tang L, Cox C, Markus MA, Luna LE, Ikeda Y, Watanabe R, Bedoya E, Yu C, Alikhan S, Wagner G, Asano K.
Biochemistry
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(1)H, (13)C, and (15)N chemical shift assignments for PfPMT, a phosphoethanolamine methyltransferase from Plasmodium falciparum.
Authors: Authors: Bezsonova I, Rujan I, Bobenchik AM, Gorbatyuk V, Maciejewski MW, Gorbatyuk O, Hao B, Arthanari H, Mamoun CB, Hoch JC.
Biomol NMR Assign
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Pulse design for broadband correlation NMR spectroscopy by multi-rotating frames.
Authors: Authors: Coote P, Arthanari H, Yu TY, Natarajan A, Wagner G, Khaneja N.
J Biomol NMR
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