Picture of Haribabu Arthanari
Haribabu Arthanari, Ph.D.
Assistant Professor of Biological Chemistry and Molecular Pharmacology

We utilize a combination of techniques including NMR spectroscopy, NMR-based fragment and high throughput screening, and biophysical and cell-based assays to map hotspots in the interaction interface, to further understand the molecular mechanisms orchestrated by these interactions, and to identify disruptive inhibitors that may be developed into treatments for the related pathologies.

Haribabu Arthanari received his Bachelors in Chemistry from the Madras Christian College and his Masters in Chemistry from the Indian Institute of Technology (IIT)-Madras.  He did his graduate studies at Wesleyan University with Philip  Bolton and earned his PhD in 2004. He then joined the laboratory of Gerhard Wagner for his post-doctoral training. He was promoted to a lecturer in 2010 and moved to his independent position in 2016.

 

Research:

Protein-Protein Interactions (PPIs) is the Holy Grail of therapeutic intervention, offering a plethora of unique structural landscapes as potential targets. I use structure-guided approaches to characterize and validate these interactions in the context of disease models.  We utilize a combination of techniques including NMR spectroscopy, NMR-based fragment and high throughput screening, and biophysical and cell-based assays to map hotspots in the interaction interface, to further understand the molecular mechanisms orchestrated by these interactions, and to identify disruptive inhibitors that may be developed into treatments for the related pathologies.  Our current areas of focus are 1) the critical interactions between transcription factors and the general transcriptional machinery, including the Mediator complex, co-activators, and remodeling factors, and 2) translation initiation machinery demonstrated to be dysregulated in cancer disease states.  We are working on making use of NMR-derived metabolomics data in the identification of novel metabolite disease markers that in combination with cellular pathway analysis can be used to identify new potential therapeutic targets.  In order to facilitate our research goals, we also work on the development of new NMR methods for fragment screening, metabolite fingerprinting and protein-ligand interaction identification.  Our work on novel pulse sequences, pulse designs, labeling strategies and sampling schemes let us push the boundaries of NMR as a technique, allowing us to tackle larger systems by NMR.

Address: 

Longwood Center

LC-3311

360 Longwood Ave.

Boston, MA 02115

Publications View
A biphenyl inhibitor of eIF4E targeting an internal binding site enables the design of cell-permeable PROTAC-degraders.
Authors: Authors: Fischer PD, Papadopoulos E, Dempersmier JM, Wang ZF, Nowak RP, Donovan KA, Kalabathula J, Gorgulla C, Junghanns PPM, Kabha E, Dimitrakakis N, Petrov OI, Mitsiades C, Ducho C, Gelev V, Fischer ES, Wagner G, Arthanari H.
Eur J Med Chem
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Local deuteration enables NMR observation of methyl groups in proteins from eukaryotic and cell-free expression systems.
Authors: Authors: Dubey A, Stoyanov N, Viennet T, Chhabra S, Elter S, Borggräfe J, Viegas A, Nowak RP, Burdziev N, Petrov O, Fischer ES, Etzkorn M, Gelev V, Arthanari H.
Angew Chem Int Ed Engl
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Hanudatta S. Atreya (1974-2020).
Authors: Authors: Arthanari H, Chary KVR, Szyperski T, Ramanathan KV, Hosur RV, Suryaprakash N, Raghothama S, Jagannathan NR, Norton RS, Prabhakaran EN, Kondaish P, Mukherjee SP, Adiga SK, Vadrevu R, Bhunia A, Joseph D.
Magn Reson Chem
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Optimization of phase dispersion enables broadband excitation without homonuclear coupling artifacts.
Authors: Authors: Coote P, Bermel W, Arthanari H.
J Magn Reson
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1H, 15N, and 13C backbone resonance assignments of the C4b-binding region from the S. aureus extracellular adherence protein.
Authors: Authors: Pal I, Mishra N, Herrera AI, Dubey A, Arthanari H, Geisbrecht BV, Prakash O.
Biomol NMR Assign
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A multi-pronged approach targeting SARS-CoV-2 proteins using ultra-large virtual screening.
Authors: Authors: Gorgulla C, Padmanabha Das KM, Leigh KE, Cespugli M, Fischer PD, Wang ZF, Tesseyre G, Pandita S, Shnapir A, Calderaio A, Gechev M, Rose A, Lewis N, Hutcheson C, Yaffe E, Luxenburg R, Herce HD, Durmaz V, Halazonetis TD, Fackeldey K, Patten JJ, Chuprina A, Dziuba I, Plekhova A, Moroz Y, Radchenko D, Tarkhanova O, Yavnyuk I, Gruber C, Yust R, Payne D, Näär AM, Namchuk MN, Davey RA, Wagner G, Kinney J, Arthanari H.
iScience
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Pre-T cell receptors topologically sample self-ligands during thymocyte ß-selection.
Authors: Authors: Li X, Mizsei R, Tan K, Mallis RJ, Duke-Cohan JS, Akitsu A, Tetteh PW, Dubey A, Hwang W, Wagner G, Lang MJ, Arthanari H, Wang JH, Reinherz EL.
Science
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Nearest-neighbor NMR spectroscopy: categorizing spectral peaks by their adjacent nuclei.
Authors: Authors: Behera SP, Dubey A, Chen WN, De Paula VS, Zhang M, Sgourakis NG, Bermel W, Wagner G, Coote PW, Arthanari H.
Nat Commun
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Allosterically coupled multi-site binding of testosterone to human serum albumin.
Authors: Authors: Jayaraj A, Schwanz HA, Spencer DJ, Bhasin S, Hamilton JA, Jayaram B, Goldman AL, Krishna M, Krishnan M, Shah A, Jin Z, Krenzel E, Nair SN, Ramesh S, Guo W, Wagner G, Arthanari H, Peng L, Lawney B, Jasuja R.
Endocrinology
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Correction to: The role of NMR in leveraging dynamics and entropy in drug design.
Authors: Authors: Dubey A, Takeuchi K, Reibarkh M, Arthanari H.
J Biomol NMR
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