Picture of Haribabu Arthanari
Haribabu Arthanari, Ph.D.
Assistant Professor of Biological Chemistry and Molecular Pharmacology

We utilize a combination of techniques including NMR spectroscopy, NMR-based fragment and high throughput screening, and biophysical and cell-based assays to map hotspots in the interaction interface, to further understand the molecular mechanisms orchestrated by these interactions, and to identify disruptive inhibitors that may be developed into treatments for the related pathologies.

Haribabu Arthanari received his Bachelors in Chemistry from the Madras Christian College and his Masters in Chemistry from the Indian Institute of Technology (IIT)-Madras.  He did his graduate studies at Wesleyan University with Philip  Bolton and earned his PhD in 2004. He then joined the laboratory of Gerhard Wagner for his post-doctoral training. He was promoted to a lecturer in 2010 and moved to his independent position in 2016.

 

Research:

Protein-Protein Interactions (PPIs) is the Holy Grail of therapeutic intervention, offering a plethora of unique structural landscapes as potential targets. I use structure-guided approaches to characterize and validate these interactions in the context of disease models.  We utilize a combination of techniques including NMR spectroscopy, NMR-based fragment and high throughput screening, and biophysical and cell-based assays to map hotspots in the interaction interface, to further understand the molecular mechanisms orchestrated by these interactions, and to identify disruptive inhibitors that may be developed into treatments for the related pathologies.  Our current areas of focus are 1) the critical interactions between transcription factors and the general transcriptional machinery, including the Mediator complex, co-activators, and remodeling factors, and 2) translation initiation machinery demonstrated to be dysregulated in cancer disease states.  We are working on making use of NMR-derived metabolomics data in the identification of novel metabolite disease markers that in combination with cellular pathway analysis can be used to identify new potential therapeutic targets.  In order to facilitate our research goals, we also work on the development of new NMR methods for fragment screening, metabolite fingerprinting and protein-ligand interaction identification.  Our work on novel pulse sequences, pulse designs, labeling strategies and sampling schemes let us push the boundaries of NMR as a technique, allowing us to tackle larger systems by NMR.

Address: 

Longwood Center

LC-3311

360 Longwood Ave.

Boston, MA 02115

Publications View
N-Acyl Homoserine Lactone Analog Modulators of the Pseudomonas aeruginosa Rhll Quorum Sensing Signal Synthase.
Authors: Authors: Shin D, Gorgulla C, Boursier ME, Rexrode N, Brown EC, Arthanari H, Blackwell HE, Nagarajan R.
ACS Chem Biol
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Emerging solution NMR methods to illuminate the structural and dynamic properties of proteins.
Authors: Authors: Arthanari H, Takeuchi K, Dubey A, Wagner G.
Curr Opin Struct Biol
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Structure determination using solution NMR: Is it worth the effort?
Authors: Authors: Takeuchi K, Baskaran K, Arthanari H.
J Magn Reson
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NMR: an essential structural tool for integrative studies of T cell development, pMHC ligand recognition and TCR mechanobiology.
Authors: Authors: Mallis RJ, Brazin KN, Duke-Cohan JS, Hwang W, Wang JH, Wagner G, Arthanari H, Lang MJ, Reinherz EL.
J Biomol NMR
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Correction to "Abietane-Type Diterpenoids Inhibit Protein Tyrosine Phosphatases by Stabilizing an Inactive Enzyme Conformation".
Authors: Authors: Hjortness MK, Riccardi L, Hongdusit A, Ruppe A, Zhao M, Kim EY, Zwart PH, Sankaran B, Arthanari H, Sousa MC, De Vivo M, Fox JM.
Biochemistry
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Backbone resonance assignments of innate immune evasion protein EapH2 from the S. aureus.
Authors: Authors: Herrera AI, Dubey A, Geisbrecht BV, Arthanari H, Prakash O.
Biomol NMR Assign
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Aromatic 19F-13C TROSY: a background-free approach to probe biomolecular structure, function, and dynamics.
Authors: Authors: Boeszoermenyi A, Chhabra S, Dubey A, Radeva DL, Burdzhiev NT, Chanev CD, Petrov OI, Gelev VM, Zhang M, Anklin C, Kovacs H, Wagner G, Kuprov I, Takeuchi K, Arthanari H.
Nat Methods
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Nonuniform Sampling for NMR Spectroscopy.
Authors: Authors: Robson S, Arthanari H, Hyberts SG, Wagner G.
Methods Enzymol
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The T Cell Antigen Receptor a Transmembrane Domain Coordinates Triggering through Regulation of Bilayer Immersion and CD3 Subunit Associations.
Authors: Authors: Brazin KN, Mallis RJ, Boeszoermenyi A, Feng Y, Yoshizawa A, Reche PA, Kaur P, Bi K, Hussey RE, Duke-Cohan JS, Song L, Wagner G, Arthanari H, Lang MJ, Reinherz EL.
Immunity
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Abietane-Type Diterpenoids Inhibit Protein Tyrosine Phosphatases by Stabilizing an Inactive Enzyme Conformation.
Authors: Authors: Hjortness MK, Riccardi L, Hongdusit A, Ruppe A, Zhao M, Kim EY, Zwart PH, Sankaran B, Arthanari H, Sousa MC, De Vivo M, Fox JM.
Biochemistry
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