Picture of Andrew Kruse
Andrew Kruse, Ph.D.
Associate Professor of Biological Chemistry and Molecular Pharmacology

Our research aims to elucidate the molecular basis of membrane protein signaling using techniques including protein engineering, structural biology, and pharmacology.

 

Research:

Signal transduction across cell membranes plays a central role in human physiology and disease, yet the mechanistic details underlying transmembrane signaling remain poorly understood. Our research aims to elucidate the molecular basis of membrane protein signaling using techniques including protein engineering, structural biology, and pharmacology. In particular, we are focused on the study of proteins important in human health and disease, including G protein-coupled receptors and other proteins that regulate neurotransmission and metabolic homeostasis.

G protein-coupled receptors (GPCRs) are cell-surface receptors that regulate neurotransmission, cardiovascular function, metabolic homeostasis, and many other physiological processes. Due to their central role in human physiology, these receptors are among the most important targets of therapeutic drugs, and are they among the most extensively studied proteins. To better understand GPCR signal transduction at a molecular level, we are using structural biology and biophysical methods to study model GPCRs such as muscarinic acetylcholine receptors. In addition, we are using new approaches in combinatorial biology to facilitate structural studies and to create protein ligands of GPCRs.

We are also interested in signal transduction pathways that remain less extensively studied than GPCRs, particularly receptors involved in the regulation of human metabolic homeostasis. In the long term, we hope to leverage our understanding of molecular signal transduction to guide the development of new and better therapeutics that modulate these pathways.

Address: 

Room SGM - 227

250 Longwood Avenue

Boston, MA 02115

Publications View
The Molecular Function of s Receptors: Past, Present, and Future.
Authors: Authors: Schmidt HR, Kruse AC.
Trends Pharmacol Sci
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A Forward Chemical Genetic Screen Reveals Gut Microbiota Metabolites That Modulate Host Physiology.
Authors: Authors: Chen H, Nwe PK, Yang Y, Rosen CE, Bielecka AA, Kuchroo M, Cline GW, Kruse AC, Ring AM, Crawford JM, Palm NW.
Cell
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FtsW is a peptidoglycan polymerase that is functional only in complex with its cognate penicillin-binding protein.
Authors: Authors: Taguchi A, Welsh MA, Marmont LS, Lee W, Sjodt M, Kruse AC, Kahne D, Bernhardt TG, Walker S.
Nat Microbiol
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Structural basis of unidirectional export of lipopolysaccharide to the cell surface.
Authors: Authors: Owens TW, Taylor RJ, Pahil KS, Bertani BR, Ruiz N, Kruse AC, Kahne D.
Nature
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Structural basis for KCTD-mediated rapid desensitization of GABAB signalling.
Authors: Authors: Zheng S, Abreu N, Levitz J, Kruse AC.
Nature
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Distinctive Activation Mechanism for Angiotensin Receptor Revealed by a Synthetic Nanobody.
Authors: Authors: Wingler LM, McMahon C, Staus DP, Lefkowitz RJ, Kruse AC.
Cell
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Solving a specificity mystery.
Authors: Authors: Zheng S, Kruse AC.
Elife
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Evidence that regulation of intramembrane proteolysis is mediated by substrate gating during sporulation in Bacillus subtilis.
Authors: Authors: Ramírez-Guadiana FH, Rodrigues CDA, Marquis KA, Campo N, Barajas-Ornelas RDC, Brock K, Marks DS, Kruse AC, Rudner DZ.
PLoS Genet
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A central role for PBP2 in the activation of peptidoglycan polymerization by the bacterial cell elongation machinery.
Authors: Authors: Rohs PDA, Buss J, Sim SI, Squyres GR, Srisuknimit V, Smith M, Cho H, Sjodt M, Kruse AC, Garner EC, Walker S, Kahne DE, Bernhardt TG.
PLoS Genet
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Structural basis for s1 receptor ligand recognition.
Authors: Authors: Schmidt HR, Betz RM, Dror RO, Kruse AC.
Nat Struct Mol Biol
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