Cryo-ET Specialist

CryoET Specialist Job Opening

The Harvard Cryo-EM Center for Structural Biology (HC2EM) is seeking a CryoET specialist to support and expand investigations of cellular and macromolecular structures using electron cryo-microscopy (cryo-EM) and cryo-electron tomography (cryoET).  The HC2EM serves Harvard Medical School, Boston Children's Hospital, Dana-Farber Cancer Institute, Massachusetts General Hospital, and a variety of external academic and commercial users (https://cryoem.hms.harvard.edu/).  The primary responsibilities of the successful candidate include ensuring high quality data is generated on a recently acquired Aquilos 2 cryo-FIB and our well-established Krios electron microscopes, training users in sample preparation and image acquisition, and providing guidance in current and future image processing programs and pipelines.  Candidates should have experience in managing and operating high-end cryo-EM equipment such as the Aquilos cryo-FIB and microscopes such as the Talos Arctica and Titan Krios, with an emphasis on cryoET methods and/or cryo-FIB. Hands-on experience with cell or tissue preparation for electron microscopy is preferable.  The candidate will work closely with a team of scientists to support projects in many research areas and will take on some facility management responsibilities. Good technical, communication, and teaching skills are essential for this position.  Salary will be commensurate with experience.

Responsibilities:

  • Serve as the technical interface between users and Aquilos 2 cryo-FIB.
  • Train users to operate TEM microscopes for tomography data collection and single particle data collection as required.
  • Advise researchers on experimental strategy and, when appropriate, collaborate with them to assist in data acquisition, analysis and structure determination (e.g. segmentation or sub-tomogram averaging).
  • Interact closely with the core facilities’ team to manage microscope and equipment maintenance, coordinate user scheduling and training, and interface with SBGrid to assist with the computational needs as new equipment comes onboard.

Benefits:

  • This position will have a high degree of independence.
  • Opportunities to present at regional and national scientific symposia and workshops.
  • Opportunities to develop collaborative relationships with Harvard community investigators.
  • Harvard employment benefits include group health, dental, disability & life insurance, subsidized public transportation passes, and subsidized fitness club memberships.

Basic Qualifications:

  • Experience in a field related to structural biology, cell biology, or biochemistry.  Ph.D. or equivalent experienced is desired.
  • Experience with cryo-EM, cryoET, and FIB-SEM.  CryoET practical application and analysis experience is valuable.

Additional Preferred Skills:

  • Must be detail-oriented and able to manage multiple projects simultaneously.
  • Good communication and interpersonal skills.

Harvard is an Affirmative Action/Equal Opportunity Employer. Applications from women and minority candidates are strongly encouraged. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, gender identity, sexual orientation, pregnancy and pregnancy-related conditions or any other characteristic protected by law.

The University requires all Harvard community members to be fully vaccinated against COVID-19 and remain up to date with COVID-19 vaccine boosters, as detailed in Harvard’s Vaccine & Booster Requirements. Individuals may claim exemption from the vaccine requirement for medical or religious reasons. More information regarding the University’s COVID vaccination requirement, exemptions, and verification of vaccination status may be found at the University’s “COVID-19 Vaccine Information” webpage: http://www.harvard.edu/coronavirus/covid-19-vaccine-information/.

To apply, please submit a cover letter, CV, and contact information for two references, to the faculty director, Dr. Stephen Harrison, via schadmin@crystal.harvard.edu. In the Subject line of your email, please write: CryoET Specialist.

 

Harvard Medical School strives to cultivate an environment that promotes inclusiveness and collaboration among students, faculty and staff and to create new avenues for discussion that will advance our shared mission to improve the health of people throughout the world.